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Microbiology practicals and calculations

Cell biology • Cell structure

Key concepts

What you'll likely be quizzed about

  • Aseptic technique prevents unwanted microorganisms from contaminating cultures and protects handlers from exposure.
  • Sterile conditions reduce competition from background microbes, allowing target organisms to grow without interference.
  • Clean benches, labelled plates, sterile inoculating loops, and minimal opening of containers form essential controls that lower the contamination risk.

Flashcards

Test your knowledge with interactive flashcards

What is a streak plate and why is it used?

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A streak plate technique spreads cells across agar to create isolated colonies for identification.

Key notes

Important points to keep in mind

Flame the loop until red-hot and allow to cool; repeat between transfers.

Label the underside of plates to avoid mix-ups.

Use light adhesive tape to secure lids; avoid full sealing.

Incubate plates upside down to prevent condensation spreading colonies.

Measure diameter at the widest point and convert to radius for area calculations using πr².

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