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Identifying plant diseases and diagnostic tests

Infection and responsePlant disease (biology only)

Flashcards

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How do monoclonal antibody test kits work?

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Monoclonal antibody kits bind to specific pathogen antigens and produce a visible signal when binding occurs, giving a rapid positive or negative result.

Key concepts

What you'll likely be quizzed about

Visible symptoms and reference guides

Plants show predictable symptoms such as stunted growth, spots, malformed leaves, discoloration and areas of decay; these symptoms guide initial identification. Gardeners and horticulturists compare symptoms with descriptions and photographs in gardening manuals or reputable websites to form a provisional diagnosis, because matching visible signs to known diseases provides a rapid first step in control and treatment.

Taking samples to a laboratory

Laboratories provide confirmatory identification through microscopic examination, culturing and biochemical tests. Culture techniques such as streak plates isolate bacterial or fungal colonies so that colony appearance and further tests identify the pathogen; accurate lab diagnosis allows targeted treatment. Limitations include time taken for cultures to grow, need for sterile technique and the possibility that some pathogens do not culture well.

Testing kits and monoclonal antibodies (Higher tier only)

Monoclonal antibodies bind specifically to antigens on pathogens and form the basis of many rapid test kits. These kits produce quick, specific positive or negative results for particular pathogens, reducing the need for time-consuming culture. Limitations include the kit’s specificity to particular pathogens (false negatives for untested pathogens) and cost; proper interpretation requires understanding of what the kit detects.

Cause → effect: identification leading to treatment

Correct identification of the causal agent (virus, bacterium, fungus, pest or nutrient deficiency) directs the appropriate control measure: antifungal spray for fungal infections, removal and burning of infected tissue to prevent spread, or nutrient amendment for deficiency. Misidentification produces ineffective treatments and wasted resources, so confirmation by laboratory tests or specific kits improves treatment success.

Practical limits and accuracy

Visual guides suit common, distinctive diseases but can mislead when symptoms overlap between causes (for example viral mosaic patterns versus fungal spots). Laboratory tests increase accuracy but require time, equipment and sterile technique. Monoclonal antibody kits are fast and specific but only detect the pathogens they are designed for; negative results do not exclude other infections.

Key notes

Important points to keep in mind

Match visible symptoms to reputable guides for a provisional diagnosis; include photos and multiple sources for comparison.

Send samples to a laboratory for confirmation when accurate identification affects treatment or biosecurity.

Use sterile sampling techniques to avoid contamination and false culture results.

Use monoclonal antibody kits for rapid, specific detection only when the target pathogen is suspected; negative results do not exclude other causes.

Consider nutrient deficiency and pest damage as alternative causes of similar symptoms and test soil or inspect for pests before applying chemical treatments.

Record symptoms, date and environmental conditions to help laboratory staff and improve diagnostic accuracy.

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